Coordinated and differential expression of histone-like proteins in Escherichia coli: regulation and function of the H-NS analog StpA.
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منابع مشابه
Role of histone-like proteins H-NS and StpA in expression of virulence determinants of uropathogenic Escherichia coli.
The histone-like protein H-NS is a global regulator in Escherichia coli that has been intensively studied in nonpathogenic strains. However, no comprehensive study on the role of H-NS and its paralogue, StpA, in gene expression in pathogenic E. coli has been carried out so far. Here, we monitored the global effects of H-NS and StpA in a uropathogenic E. coli isolate by using DNA arrays. Express...
متن کاملRegulation of lysyl-tRNA synthetase expression by histone-like protein H-NS of Escherichia coli.
The lysU gene encoding lysyl-tRNA synthetase of Escherichia coli is normally silent at low temperatures and is expressed by certain metabolites and stimuli. A novel class of lysU-constitutive mutations were isolated by random insertion mutagenesis. These mutations nullified the hns gene encoding a histone-like protein, H-NS, and affected thermoregulation of lysU.
متن کاملDifferential dependence of StpA on H-NS in autoregulation of stpA and in regulation of bgl.
StpA has functional similarity to its homologue, the nucleoid structuring protein H-NS. It binds to AT-rich, planar, bent DNA and constrains DNA supercoils. In addition, StpA acts as an RNA chaperone. StpA and H-NS also form heterodimers. However, cellular levels of StpA are low due to repression of stpA by H-NS and negative autoregulation. Here we show that effective (30-fold) repression of st...
متن کاملProbing the structure, function, and interactions of the Escherichia coli H-NS and StpA proteins by using dominant negative derivatives.
Twelve different dominant negative mutants of the Escherichia coli nucleoid-associated protein, H-NS, have been selected and characterized in vivo. The mutants are all severely defective in promoter repression activity in a strain lacking H-NS, and they all disrupt the repression normally exerted by H-NS at two of its target promoters. From the locations of the alterations in these mutants, whi...
متن کاملNucleotide sequence of a newly-identified Escherichia coli gene, stpA, encoding an H-NS-like protein.
analysis of a 978-bp fragment of this segment revealed an ORF , w . r o , * r. A A , ^ c ,,noo> ^ * o i«> M.-> , J , r . b r IIA • J /i-.N 1Martinez,E., Bartolomi.B. and de la Cruz,F. (1988) Gene 68, 159-162. that encodes a putative protein of 134 ammo acids (Figure 1). 2 DevereuxJ., Haeberli.P. and Smithies.O. (1984) Nucleic Acids Res. 12, Comparative searches (FASTA, TFASTA (2)) showed that t...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1996
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1996.tb00877.x